Regenerative Medicine of Cardiovascular Diseases

(Deutsch: Lehrstuhl für Regenerative Medizin kardiovaskulärer Erkrankungen)


Regenerative therapies, either by transplantation of stem cells or stem cell-derived differentiated cells into the defective organ or by promoting endogenous regenerative processes, are an important goal of cardiovascular research. However, to successfully translate these concepts into patient care, several fundamental research questions have to be answered, and many problems have to be solved. One core activity of this group is to address such questions with state-of-the-art methods.


Research

Cardiovascular disorders account today for about 30% of deaths world wide. An urgent need in cardiovascular medicine is to develop novel therapeutic tools and approaches to restore proper function of diseased hearts and to regenerate cardiac muscle after injury. Latest discoveries and advanced knowledge in the fields of stem cell biology, developmental cardiology, and genome editing hold great promise for achieving this goal. 


Methods

  • Generation, culture and differentiation of human pluripotent stem cells
  • Genetic modification (CRISPR/Cas system)
  • Optical physiological characterization of cardiomyocytes (Calcium imaging, membrane potential imaging, second messenger imaging)
  • Three-dimensional culture of iPS-derived cardiomyocytes enabling measurements of contractility and intracellular calcium
  • Gene expression analysis (RNAseq, single-cell RNAseq)
  • Molecular cloning, routine molecular biology methods
  • Western blot, immune fluorescence, co-immuneprecipitation
  • Cell injection into murine embryos (in utero and ex utero)